Guido's guardians are supposedly Uncle Topolino and Mama Topolino, and possibly Mama Luigi as well, though it remains unconfirmed if they truly are. In Tokyo Mater, Luigi is hanging out at Flo's V8 Café before DJ, Boost and Wingo disturb them, causing Mater to begin his story about how he was once a drift car. He has also been shown to be on good terms with the rest of the Radiator Springs residents, particularly Lightning McQueen. Great variety of characters, from the most popular of all time to the most modern, which is extended several times a year. Guido is one of the six playable characters, the other five being Lightning McQueen, Cruz Ramirez, Mater, Sally, and Ramone, who are available from the start in Cars 3: Driven to Win.

In Disney Infinity, Luigi appears as a cast member in the Cars Play Set, as well as an opponent during race challenges. Guido was headed to the United States on a cruise ship to pursue his dream of being on a real racing crew, when he overheard Luigi talking about Ferrari to a nearby passenger.

A car of few English words other than "pit stop," Guido is a force to be reckoned with when he's changing tires in the pits during a race. In Rescue Squad Mater, he watches Guido balancing a tire on top of a few more tires, and his attention is caught by the sight of Nurse GTO. To better prepare himself, he practices tire changes at night on wooden practice frames he built in his garage. J. Holowicki • Elissa Knight • Lindsey Collins • Larry Benton • Douglas Keever • Tom Hanks • Tim Allen • Billy Crystal • John Goodman • Dave Foley • Rodger Bumpass • Artie Kempner • Hooman Khalili • Jess Harnell • Sonoko Konishi • Sheryl Crow • Jack Angel • Michael Bell • Bob Bergen • Susan Blu • Andrea Boerries • Marco Boerries • Torbin Xan Bullock • Richard Cawood • Scott Clark • Kathy Coates • Jennifer Darling • Paul Eiding • Bill Farmer • Brian Fee • Teresa Ganzel • Craig Good • Erik Langley • Sherry Lynn • Danny Mann • Mickie McGowan • Laraine Newman • Teddy Newton • Colleen O' Shaughnessey • Bob Peterson • Steve Purcell • A.

They have known each other since their early days in Italy, where they were known to fight a lot, though continued to remain best friends.

Additionally, a Toy Box costume version of him can be unlocked in the Toy Box, and being one of the townspeople who give tutorials about the Toy Box.